Induction of infectious petunia vein clearing (pararetro) virus from endogenous provirus in petunia

Analysis of Petunia vein clearing virus (PVCV) sequences, retroelements and tandem repeats in Petunia axillaris N and P. inflata S6

Petunia vein-clearing virus: a plant pararetrovirus with the core sequences for an integrase function.

Petunia vein-clearing virus (PVCV) is a plant pararetrovirus that has some features of retrotransposons. It encapsidates dsDNA and has isometric particles and inclusion bodies similar to those of caulimoviruses. The PVCV genome of 7205 bp has two large ORFs in the transcribed strand and a methionine tRNA primer-binding site in its 663-bp intergenic region. The N-terminal position of the large p...

Undesired Small RNAs Originate from an Artificial microRNA Precursor in Transgenic Petunia (Petunia hybrida)

Although artificial microRNA (amiRNA) technology has been used frequently in gene silencing in plants, little research has been devoted to investigating the accuracy of amiRNA precursor processing. In this work, amiRNAchs1 (amiRchs1), based on the Arabidopsis miR319a precursor, was expressed in order to suppress the expression of CHS genes in petunia. The transgenic plants showed the CHS gene-s...

Isolation barriers between petunia axillaris and Petunia integrifolia (Solanaceae).

The isolation barriers restricting gene flow between populations or species are of crucial interest for understanding how biological species arise and how they are maintained. Few studies have examined the entire range of possible isolation barriers from geographic isolation to next generation hybrid viability. Here, we present a detailed analysis of isolation barriers between two flowering pla...

Effects of Glycerol and Salicylic Acid on Freezing Tolerance of Iranian Petunia (Petunia hybrida L.)

The aim of this study was to investigate the effects of glycerol (GL) and salicylic acid (SA) on freezing tolerance of Iranian Petunia (Petunia hybrida). For this purpose, a factorial experiment based on completely randomized design with three replications was conducted under controlled conditions. The first factor was temperature (0 and -4 °C) and the second factor was spraying with anti-stres...